Marcus J. Claesson
School of Microbiology and APC Microbiome Institute, University College Cork, Cork, Ireland
Thursday, 29 December 2016
LIBRARY GENERATION THROUGH RIBOSOMAL RNA 16S GENE AMPLIFICATION FOR DETERMINING MICROBIOTA COMPOSITION
Many metagenomic studies are aimed at determining the bacterial composition in a specific type of sample. This is usually performed by next-generation sequencing analysis of the data obtained from libraries created by PCR amplification of the prokaryotic 16S ribosomal RNA (rRNA) gene. This gene has a length of 1542 base pairs and it is composed by nine variable regions and interspaced by more conserved regions. The amplification of one or two of these variable regions of the 16S rRNA gene is a common approach employed in the phylogenetic identification of hundreds of species that are present in the human microbiota1.