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Volumen11

Monday, 30 September 2019

LÍNEA EDITORIAL DE NUESTRA REVISTA

Written by Editores de la revista, Posted in Volumen11

¿La curiosidad mató al gato?

LÍNEA EDITORIAL DE NUESTRA REVISTA
En nuestra profesión, la curiosidad científica es algo inherente y casi imprescindible para disfrutar de su ejercicio. El deseo de averiguar la causa de algo que no entra dentro de la normalidad, constituye el motor que nos permite abandonar nuestros límites, dispuestos a explorar y conocer más sobre el tema en busca de una respuesta.
 
El laboratorio nos ofrece la oportunidad de utilizar la curiosidad a modo de herramienta para encontrar respuestas y a la vez expandir el conocimiento y experiencia adquiridos a nuestro entorno.

Monday, 30 September 2019

ARTRITIS GOTOSA POR CRISTALES DE URATO MONOSÓDICO

Written by Arturo González Raya | Ramón Coca Zúñiga | Fernando Miguel Cantero Sánchez, Posted in Volumen11

ARTRITIS GOTOSA POR CRISTALES DE URATO MONOSÓDICO
Figure 1. Example of gout crystals (monosodium urate crystals) visualized (40x objective) under polarized light with red compensator (black arrow indicates the axis of the compensator).

Synovial fluid (SF), is a dialysate of plasma that internally covers the cavities of synovial joints. It is formed by ultrafiltration of plasma in the synovial capillaries.

There are two types of cells on the synovial lining known as type "A" and "B" synoviocytes. The type A cells have phagocytic functions while the type B cells with secretion functions, add to the SF among other substances, mucopolysaccharides with high hyaluronate content that gives it the characteristic viscosity.

Monday, 30 September 2019

CUERPOS DE PAPPENHEIMER EN MORFOLOGÍA DE SANGRE PERIFÉRICA

Written by Luiza Tofan | Carlos Navarro Morante | Óscar Fuster Lluch , Posted in Volumen11

CUERPOS DE PAPPENHEIMER EN MORFOLOGÍA DE SANGRE PERIFÉRICA
Figure 1. Pappenheimer bodies in erythroblast and in hemocyte (Blood smear. May-Grünwald Giemsa stain).
We present an image of a peripheral blood smear, stained with May-Grünwald Giemsa (Figure 1). This picture shows erythrocytes with multiple inclusions of bluish, small and irregular granules that occupy a portion of the red blood cell. The inclusions described are also observed in erythroblasts, adopting a perinuclear disposition. These granules, suggestive of Pappenheimer bodies, are morphologically different from other visible inclusions with the same type of staining (Howell-Jolly and basophilic stippling). This corresponds to intracellular iron deposits which were evidenced later by Perls staining (Figure 2).

Monday, 30 September 2019

IMPORTANCIA DEL DIAGNÓSTICO MOLECULAR RÁPIDO PARA ENFERMEDADES GENÉTICAS CON TRATAMIENTO

Written by Irene Mª Pérez Lucendo | Virginia Moreno Moral | Antonio Martínez Peinado, Posted in Volumen11

IMPORTANCIA DEL DIAGNÓSTICO MOLECULAR RÁPIDO PARA ENFERMEDADES GENÉTICAS CON TRATAMIENTO
Figure 1. Result of MLPA of the patient´s DNA with suspected AME type I. A homozygous deletion (exons 7 and 8) is observed of SMN1 gene (0 copies), as well as four copies for the SMN2 gene. Thus, the diagnostic suspicion is confirmed.

It is presented the diagnosis of Spinal Muscular Atrophy (AME in Spanish language) performed in the Molecular Genetics laboratory by means of an MLPA study (Multiplex Ligations Probe Amplification) by SALSA MLPA-P060-B2 (MRC-Holland®, Netherlands), furthermore SALSA MLPA-P021 is used to confirm the diagnosis(1). This method allows us to know the copy number of the survival genes of the motor neuron (SMN1 and SMN2) in a fast and reliable way (95% sensitivity and 100% specificity).
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